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Tissue was stained using the Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). 2002-11-01 Distribution of cadherin types in the rat anterior pituitary was examined immunohistochemically to clarify the unique cell arrangements caused by homologous and heterologous affinities among cells. N‐ and E‐cadherins were detected continuously along cell membranes, while P‐cadherin was not. N-cadherin, also known as Cadherin-2 (CDH2) or neural cadherin (NCAD) is a protein that in humans is encoded by the CDH2 gene. CDH2 has also been designated as CD325 (cluster of differentiation 325). N-cadherin is a transmembrane protein expressed in multiple tissues and functions to mediate cell–cell adhesion.

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anticoagulant : / assay procedure step 1 : 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml. background : Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. 2011-12-13 In the present paper, we characterize an antibody, mAb BV13, directed to mouse vascular endothelial (VE)-cadherin, a major adhesive protein of interendothelial adherens junctions. When added to cultured endothelial cells, BV13 induces a redistribution of VE-cadherin from intercellular junctions.

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Summary Other designations. cadherin-5, VE-cadherin, vascular N‑Cadherin in A549 Human Cell Line. N-Cadherin was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human/Mouse/Rat N-Cadherin Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 10 µg/mL for 3 hours at room temperature. 2017-01-09 · N-cadherin Antibody (D-4) is a high quality monoclonal N-cadherin antibody (also designated NCAD antibody, CD325 antibody or CDH2 antibody) suitable for the detection of the N-cadherin protein of mouse, rat and human origin.

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ED 50 for this effect is 0.3 – 1.2 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells. Stability testing for Rat E-Cadherin. Rat E-Cadherin was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. In this study, anti-rat pan-cadherin antibody, specific to all subgroups of the cadherin family, was used to map the distribution of the pan-cadherin immunoreactivity in adult rat organs.

N‐ and E‐cadherins were detected continuously along cell membranes, while P‐cadherin was not. N-cadherin, also known as Cadherin-2 (CDH2) or neural cadherin (NCAD) is a protein that in humans is encoded by the CDH2 gene. CDH2 has also been designated as CD325 (cluster of differentiation 325). N-cadherin is a transmembrane protein expressed in multiple tissues and functions to mediate cell–cell adhesion. In cardiac muscle, N-cadherin is an integral component in adherens junctions 1999-08-14 Cadherin‑6/KCAD in MG‑63 Human Cell Line.
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21 Jun 2018 a CYP26B1 deficient human and rats with hypervitaminosis A to further Clinical Pharmacogenomics and Osteoporosis, Rudbecklaboratoriet, C11, reduced VE- Cadherin staining between endothelial cells. n = 4/treatment. K-cadherin (2B6) Antibody Santa Cruz Biotechnology catalog: sc-59974 total number of products: 4. mouse monoclonal (2B6) reactivity: human, rat application :  av C Koskinen — factors including osteotropic hormones (e.g. intermittent PTH, D3, sex steroids) and neuropeptides discovered in 1996 in rat fibroblasts as a binding protein for Src-homology-2 (2012). "N-cadherin adherens junctions mediate osteogenesis​  av M Larsson · 2006 · Citerat av 1 — The full-length sequence of rat gp330 was published been suggested for the VLDL-receptor, ApoER2 and cadherin-like neuronal receptors  receptor expressed in rat prostate and ovary.

Key Words: Osteoblasts; cell thelial-mesenchymal interactions in initiation of osteo- genesis. Int J Dev Biol Marie PJ (2002) Role of N-cadherin in bone forma- tion. 21 Jun 2018 a CYP26B1 deficient human and rats with hypervitaminosis A to further Clinical Pharmacogenomics and Osteoporosis, Rudbecklaboratoriet, C11, reduced VE- Cadherin staining between endothelial cells. n = 4/treatment. K-cadherin (2B6) Antibody Santa Cruz Biotechnology catalog: sc-59974 total number of products: 4. mouse monoclonal (2B6) reactivity: human, rat application :  av C Koskinen — factors including osteotropic hormones (e.g. intermittent PTH, D3, sex steroids) and neuropeptides discovered in 1996 in rat fibroblasts as a binding protein for Src-homology-2 (2012).
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In this study, anti-rat pan-cadherin antibody, specific to all subgroups of the cadherin family, was used to map the distribution of the pan-cadherin immunoreactivity in adult rat organs. Pan-cadherin immunoreactivity positive tissues were: secretory cells of the adenohypophysis, autonomic nerve, corneal epithelium, oesophageal nerve plexus Sandwich High Sensitivity ELISA kit for Quantitative Detection of rat Cadherin-4/R-Cadherin. 96wells/kit, with removable strips. Cite This Product Rat R-Cadherin-4 Cdh4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2095) The pEGFPN1-E-cadherin expression plasmid, which contained the rat E-cadherin-coding sequence with insertion of green fluorescent protein (GFP) at the C-terminal, was transiently transfected into endplate chondrocytes using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). At 24 h post-transfection, the cells were exposed to mechanical loading.

Exp. Date: See product label . Rat anti-E-Cadherin (Mouse) FORM . This monoclonal antibody is supplied lyophilized in phosphate-buffered saline, containing 1.0% bovine serum albumin. 10X Rat E-Cadherin Capture Antibody 600 µL +2-8ºC 10X Rat E-Cadherin Detector Antibody 600 µL +2-8ºC Rat E-Cadherin Lyophilized Recombinant Protein 2 Vials +2-8ºC Antibody Diluent 5BC 6 mL +2-8ºC 10X Wash Buffer PT 20 mL +2-8ºC TMB Substrate 12 mL +2-8ºC Stop Solution 12 mL +2-8ºC Sample Diluent NS* 50 mL +2-8ºC View protein in InterPro IPR039808, Cadherin IPR002126, Cadherin-like_dom IPR015919, Cadherin-like_sf IPR020894, Cadherin_CS IPR000233, Cadherin_cytoplasmic-dom IPR027397, Catenin-bd_sf: PANTHER i: PTHR24027, PTHR24027, 1 hit: Pfam i: View protein in Pfam PF00028, Cadherin, 5 hits PF01049, Cadherin_C, 1 hit Immobilized Recombinant Rat E-Cadherin supports the adhesion of MCF-7 cells in a dose dependent manner.
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The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous (reviewed in ref 13).